CHES, as buffer of enzyme reaction, shows high affinity to the iodoacetate binding site of HDH, it could protect the enzyme from inactivation by iodoacetic acid. Phosphodiesterase has greatest activity in CHES comparing with other buffers.
The buffer range of MOPS is 6.5-7.9. MOPS is suitable for electron transfer and phosphorylation of chloroplast thin layer preparation. It is also applicable to Northern hybridization It is used as buffer for RNA separation and
HEPES is a hydrogen ion buffer that controls a constant pH range for long periods of time. It is used to be 3'-terminal labeling for ligase of RNA and T4RNA. It is used in biochemical diagnostic kits, DNA/RNA extraction kits and PCR diagnostic kits. No toxic effects on cells.